recombinant gro α  (R&D Systems)

Journal: PLOS ONE

Article Title: Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

doi: 10.1371/journal.pone.0313693

Figure Lengend Snippet: Proteins secreted from the medium were analyzed using a RayBio Biotin Label-based Human Antibody Array. (A) Increased spot intensity for GRO-α was observed in cells derived from the same donor. The selection of intersecting proteins among candidate proteins secreted by hWJ-MSCs was higher than that of hPL-MSCs. (B) The conditioned media was collected from C2C12, hWJ-MSCs alone, hPL-MSCs alone, and co-cultured cells. The concentration of secreted GRO-α in each conditioned medium was measured using the GRO-α ELISA kit (** p < 0.01, * p < 0.05, n = 4). Data are significantly different from that in the corresponding control group.

Article Snippet: The Human GRO-α Quantikine ELISA kit (#DGR00B; R&D systems, USA) was used to analyze the expression of proteins selected from the antibody array.

Techniques: Ab Array, Derivative Assay, Selection, Cell Culture, Concentration Assay, Enzyme-linked Immunosorbent Assay, Control

Journal: PLOS ONE

Article Title: Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

doi: 10.1371/journal.pone.0313693

Figure Lengend Snippet: In vitro damaged C2C12 cells were co-cultured with hWJ-MSCs pretreated with GRO-α siRNAs, which reduced the levels of secreted GRO-α. (A) To examine knockdown of GRO-α in hWJ-MSCs, the medium was collected and GRO-α levels were analyzed using ELISA (*** p <0.001, n = 3). (B) Harvested cells were analyzed through western blotting with specific antibodies (p-RIP3, p-MLKL, β-actin). (C, D) All bands were analyzed through densitometry (** p <0.01, * p < 0.05, n = 3).

Article Snippet: The Human GRO-α Quantikine ELISA kit (#DGR00B; R&D systems, USA) was used to analyze the expression of proteins selected from the antibody array.

Techniques: In Vitro, Cell Culture, Knockdown, Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Acta biochimica et biophysica Sinica

Article Title: NLRP3 inflammasome-mediated disruption of mitochondrial homeostasis in alveolar macrophages contributes to ozone-induced acute lung inflammatory injury.

doi: 10.3724/abbs.2024171

Figure Lengend Snippet: Figure 1. O3 exposure increases lung inflammatory injury by increasing the number of alveolar macrophages and AM-related chemokines (A) HE and MT staining of mouse lung tissue. Bronchial epithelial inflammatory cell infiltration and thickening are indicated by black arrows, and collagen deposition is indicated by green arrows. Scale bar: 100 μm. (B) Total leukocyte count in the BALF. (C) Total alveolar macrophage count in the BALF. (D) Total protein level in the BALF. (E‒G) Protein levels of TNF-α, CXCL1, and MCP-1 in the BALF of the mice. *P<0.05.

Article Snippet: The levels of tumor necrosis factor-α (TNF-α), chemokine CXCL1, and the macrophage chemotactic protein MCP1 were measured using the corresponding ELISA kits (Cusabio Biotech, Wuhan, China).

Techniques: Staining

Journal: Acta biochimica et biophysica Sinica

Article Title: NLRP3 inflammasome-mediated disruption of mitochondrial homeostasis in alveolar macrophages contributes to ozone-induced acute lung inflammatory injury.

doi: 10.3724/abbs.2024171

Figure Lengend Snippet: Figure 5. Acute inflammatory injury is alleviated in NLRP3‒/‒ and Caspase-1‒/‒ mice after O3 exposure (A) Evaluation of airway resistance (a), tissue damping (b) and tissue elastance (c). (B) HE and MT staining of the lung tissue of the mice. Bronchial epithelial inflammatory cell infiltration and thickening are indicated by black arrows, and collagen deposition is indicated by green arrows. Scale bar: 100 μm. (C) Total cell count in the BALF. (D) Total alveolar macrophage count in the BALF. (E) Total protein level in the BALF. (F‒H) Protein levels of TNF-α, CXCL1, and MCP-1 in the BALF of the mice. *P<0.05.

Article Snippet: The levels of tumor necrosis factor-α (TNF-α), chemokine CXCL1, and the macrophage chemotactic protein MCP1 were measured using the corresponding ELISA kits (Cusabio Biotech, Wuhan, China).

Techniques: Staining, Cell Counting